Journal: Current protocols

Article Title: O-GlcNAc engineering on a target protein in cells with nanobody-OGT and -splitOGA

doi: 10.1002/cpz1.117

Figure Lengend Snippet: Number of wells and DNA amounts can be scaled up accordingly for additional replicates or material.

Article Snippet: Materials 1 pcDNA3.1-GFP-Flag-JunB-EPEA (Addgene, cat. No. 160743).

Techniques: Plasmid Preparation

Journal: Current protocols

Article Title: O-GlcNAc engineering on a target protein in cells with nanobody-OGT and -splitOGA

doi: 10.1002/cpz1.117

Figure Lengend Snippet: The mixture is incubated in a thermocycler with the conditions described below

Article Snippet: Materials 1 pcDNA3.1-GFP-Flag-JunB-EPEA (Addgene, cat. No. 160743).

Techniques: Incubation

Journal: Current protocols

Article Title: O-GlcNAc engineering on a target protein in cells with nanobody-OGT and -splitOGA

doi: 10.1002/cpz1.117

Figure Lengend Snippet: Number of wells and DNA amounts can be scaled up accordingly for additional replicates or material.

Article Snippet: 1 1 pcDNA3.1-GFP-Flag-JunB-EPEA (Addgene, cat. No. 160743).

Techniques: Plasmid Preparation

Journal: Current protocols

Article Title: O-GlcNAc engineering on a target protein in cells with nanobody-OGT and -splitOGA

doi: 10.1002/cpz1.117

Figure Lengend Snippet: The mixture is incubated in a thermocycler with the conditions described below

Article Snippet: 1 1 pcDNA3.1-GFP-Flag-JunB-EPEA (Addgene, cat. No. 160743).

Techniques: Incubation

Journal: Aging (Albany NY)

Article Title: Targeting regulation of ATP synthase 5 alpha/beta dimerization alleviates senescence

doi: 10.18632/aging.203858

Figure Lengend Snippet: Maintenance of 14–3–3ζ expression is a prerequisite for KB1541-mediated senescence alleviation. ( A ) Expression levels of 14–3–3ζ and ATP synthase 5 alpha/beta proteins in young and senescent fibroblasts. ( B ) shRNA-mediated 14–3–3ζ knockdown significantly reduced the endogenous expression level of 14–3–3ζ mRNA. ** P < 0.01, student t -test. Mean ± S.D., n = 3. ( C ) Immunoblot of total lysates from senescent cells transduced with lenti-virus expressing control shRNA or 14–3–3ζ shRNA. ( D ) Quantification of SA-β gal positive cells. * P < 0.05, student t -test. Mean ± S.D., n = 3.

Article Snippet: HEK 293T cells were transfected with 10 μg shRNA plasmid (control shRNA or 14–3–3ζ shRNA (sc-156019-SH; Santa Cruz Biotechnology), 5 μg PAX2 plasmid, and 5 μg VSV-G plasmid using Lipofectamine 2000 (11668019; Invitrogen).

Techniques: Expressing, shRNA, Knockdown, Western Blot, Transduction, Virus, Control